汉弥尔顿·史密斯(Hamilton O. Smith,1931年8月23日—2025年10月25日)是美国微生物学家,以分离并鉴定第一个II型限制性内切酶HindII著称,1978年与沃纳·亚伯、丹尼尔·那森斯共享诺贝尔生理学或医学奖;该发现为重组DNA技术奠定了基础。
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